Accumulation of immunoglobulin messenger ribonucleic acid in immunized mouse spleen

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Abstract
The concentration of mRNA coding for immunoglobulins [Ig], and .kappa. type L chains and .gamma.1 type H chain, in mouse spleen cells activated by bacterial [Escherichia coli] lipopolysaccharide [LPS] or sheep red blood cells. These mRNA were quantitated by hybridization to radioactive DNA complementary to highly purified Ig mRNA from mouse myelomas. In the LS-stimulated spleen cells, only L chain mRNA accumulated, whereas .gamma.1 type H chain mRNA remained unvaried. The L chain mRNA concentration also increased in purified bone-marrow-derived [B] lymphocytes. The LS-induced L chain mRNA was similar to L chain mRNAs purified from myelomas. The accumulation and disappearance of L chain mRNA in B lymphocytes coincide with the kinetics of synthesis of IgM which is the major species induced by LPS. In sheep red blood cell stimulated spleen, the specific accumulation of .kappa. type L chain and .gamma.1 type H chain mRNA parallels IgG synthesis. The increment of Ig mRNA concentration in B lymphocytes is probably important for induction of Ig synthesis.