A DNA-binding protein is required for termination of transcription by RNA polymerase I in Xenopus laevis.
Open Access
- 1 June 1990
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 10 (6) , 2793-2800
- https://doi.org/10.1128/mcb.10.6.2793
Abstract
We describe a partially fractionated in vitro transcription system from Xenopus laevis for the assay of transcription termination by RNA polymerase I. Termination in vitro was found to require a specific terminator sequence in the DNA and a DNA-binding protein fraction that produces a footprint over the terminator sequence.This publication has 20 references indexed in Scilit:
- A point mutation uncouples RNA 3'-end formation and termination during ribosomal gene transcription in Xenopus laevis.Genes & Development, 1990
- The Xenopus ribosomal gene enhancers bind an essential polymerase I transcription factor, xUBF.Genes & Development, 1989
- Termination of transcription in human mitochondria: Identification and purification of a DNA binding protein factor that promotes terminationCell, 1989
- Ribosomal precursor 3′ end formation requires a conserved element upstream of the promoterCell, 1987
- A transcriptional terminator is a novel element of the promoter of the mouse ribosomal RNA geneCell, 1986
- TRANSCRIPTION TERMINATION AND THE REGULATION OF GENE EXPRESSIONAnnual Review of Biochemistry, 1986
- A repeated 18 bp sequence motif in the mouse rDNA spacer mediates binding of a nuclear factor and transcription terminationCell, 1986
- Characterization of three sites of RNA 3$prime; end formation in the Xenopus ribosomal gene spacerCell, 1986
- Transcription of mouse rDNA terminates downstream of the 3′ end of 28S RNA and involves interaction of factors with repeated sequences in the 3′ spacerCell, 1985
- A transcriptional function for the repetitive ribosomal spacer in Xenopus laevisNature, 1983