Interaction of lymphocytes with lipid bilayer membranes: a model for lymphocyte-mediated lysis of target cells.
- 1 July 1975
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (7) , 2789-2793
- https://doi.org/10.1073/pnas.72.7.2789
Abstract
Horizontal lipid bilayer membranes were used as a model system to study lymphocyte-mediated killing of target cells. Dinitrophenylated lipid bilayers can physically support dozens of lymphocytes for periods of over one hour without breakage or increasing the electrical conductance of the membrane. However, in the presence of antibody against Dnp, human lymphocytes rapidly induced increases in membrane conductance of several orders of magnitude without membrane breakage. Such ionic permeability increases occurred only when the membrane voluage was positive on the lymphocyte side, as would be the case with a target cell membrane. The lymphocyte and antibody dependence of this conductance increase parallels that observed for lymphocyte killing of antibody-coated target cells. The results are interpreted as evidence that the primary event in lymphocyte killing of antibody-coated target cells is the creation of ion-conducting channels in the target membrane.Keywords
This publication has 14 references indexed in Scilit:
- Ultrastructure of membrane lesions in immune lysis, osmotic lysis and drug-induced lysis.1974
- STUDIES OF THE HUMAN LYMPHOCYTE RECEPTOR FOR HEAT-AGGREGATED OR ANTIGEN-COMPLEXED IMMUNOGLOBULINThe Journal of Experimental Medicine, 1974
- Estimation of the size of a T-cell-induced lytic lesionNature, 1974
- Interruption of the Sequential Release of Small and Large Molecules from Tumor Cells by Low Temperature During Cytolysis Mediated by Immune T-Cells or ComplementProceedings of the National Academy of Sciences, 1974
- Cell-Mediated Cytotoxicity, Allograft Rejection, and Tumor ImmunityPublished by Elsevier ,1974
- Studies on the mechanism of lymphocyte-mediated cytolysis. II. The use of various target cell markers to study cytolytic events.1973
- Active vs. passive sensitization of liposomes toward antibody and complement by dinitrophenylated derivatives of phosphatidylethanolamineBiochemistry, 1972
- Resistance Changes in Lipid Bilayers: Immunological ApplicationsScience, 1968
- Target cell destruction.1968
- Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.1968