Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli.
- 1 April 1998
- journal article
- Vol. 180 (8) , 2063-71
Abstract
Replacement of Escherichia coli's RecBCD function with phage lambda's Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is highlighted by gene replacement with a PCR-generated DNA fragment. The deltarecBCD::Plac-red kan replacement allele can be P1 transduced to other E. coli strains, making the hyper-Rec phenotype easily transferable.This publication has 40 references indexed in Scilit:
- A general system for generating unlabelled gene replacements in bacterial chromosomesMolecular Genetics and Genomics, 1996
- Bacteriophage P22 accessory recombination functionVirology, 1991
- Double-chain-cut sites are recombination hotspots in the red pathway of phage λJournal of Molecular Biology, 1987
- In phage λ, cos is a recombinator in the red pathwayJournal of Molecular Biology, 1985
- λ red-dependent growth and recombination of phage P22Virology, 1984
- Genetic analysis of the erf region of the bacteriophage P22 chromosomeVirology, 1984
- Insertion mutations in the dam gene of Escherichia coli K-12Molecular Genetics and Genomics, 1983
- Use of solubilizable acrylamide disulfide gels for isolation of DNA fragments suitable for sequence analysisAnalytical Biochemistry, 1981
- Gene regulation at the right operator (OR) of bacteriophage λJournal of Molecular Biology, 1980
- Rec-mediated recombinational hot spot activity in bacteriophage lambda: III. Chi mutations are site-mutations stimulating Rec-mediated recombinationJournal of Molecular Biology, 1975