The effect of aminoacyl- or peptidyl-tRNA at the A-site on the arrangement of deacylated tRNA at the ribosomal P-site
- 21 May 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 170 (2) , 290-294
- https://doi.org/10.1016/0014-5793(84)81330-7
Abstract
Photoreactive derivatives of E. coli tRNAPhe bearing arylazido groups on guanine residues (azido-tRNA) were used for affinity labelling of E. coli ribosomes in the region of the P-site when the A-site was either free or occupied by aminoacyl- or peptidyl-tRNA. Corresponding complexes of azido-tRNA with ribosomes and poly(U) were obtained both nonenzymatically and with the use of elongation factors. UV-irradiation of the complexes resulted in labelling of ribosomal proteins (preferentially of 30 S subunit). Proteins S9 and S21 were labelled only when the A-site was free; S14 — only when it was occupied; S11, S13, S19 — in both cases; S5, S7, S12, S20 — in some statesKeywords
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