The γ-subunit of the rod photoreceptor cGMP-binding cGMP-specific PDE is expressed in mouse lung
- 1 February 1998
- journal article
- Published by Springer Nature in Cell Biochemistry and Biophysics
- Vol. 29 (1) , 133-144
- https://doi.org/10.1007/bf02737832
Abstract
The type 6 phosphodiesterase (PDE-6) from retinal rod photoreceptors is an αβγ[in2] heterotetramer. The α-and β-subunits contain catalytic sites for cGMP hydrolysis, whereas the γ-subunits (Pγ) serve as a protein inhibitor of the enzyme. Pγ is believed to be expressed only in photoreceptors. Using RT-PCR, we have amplified the complete coding sequence for Pγ from mouse lung RNA. The expression of Pγ in this tissue may be related to its ability to interact the type 5 phosphodiesterase (PDE-5), which is the predominant cGMP binding protein in lung. We therefore suggest that Pγ may have a wider signaling role in mammalian cells than previousl y appreciated.Keywords
This publication has 13 references indexed in Scilit:
- The Regulation of the cGMP-binding cGMP Phosphodiesterase by Proteins That Are Immunologically Related to γ Subunit of the Photoreceptor cGMP PhosphodiesteraseJournal of Biological Chemistry, 1997
- Interaction of the catalytic subunit of protein kinase A with the lung type V cyclic GMP phosphodiesterase: Modulation of non-catalytic binding sitesBiochemical and Biophysical Research Communications, 1992
- Functional regions of the inhibitory subunit of retinal rod cGMP phosphodiesterase identified by site-specific mutagenesis and fluorescence spectroscopyBiochemistry, 1992
- The catalytic subunit of protein kinase A triggers activation of the type V cyclic GMP-specific phosphodiesterase from guinea-pig lungBiochemical Journal, 1992
- Subunit stoichiometry of retinal rod cGMP phosphodiesteraseBiochemistry, 1990
- Expression in bacteria of functional inhibitory subunit of retinal rod cGMP phosphodiesterase.Proceedings of the National Academy of Sciences, 1989
- Molecular mechanism of visual transductionEuropean Journal of Biochemistry, 1989
- [65] Purification of cGMP-binding protein phosphodiesterase from rat lungPublished by Elsevier ,1988
- Identification and characterization of both the cytosolic and particulate forms of cyclic GMP-stimulated cyclic AMP phosphodiesterase from rat liverBiochemical Journal, 1986
- Identification of cGMP-stimulated cyclic nucleotide phosphodiesterase in lung tissue with monoclonal antibodies.Journal of Biological Chemistry, 1982