Lysine-Ketoglutarate Reductase Activity in Developing Maize Endosperm
Open Access
- 1 April 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 69 (4) , 988-989
- https://doi.org/10.1104/pp.69.4.988
Abstract
Lysine-ketoglutarate reductase activity was detected and characterized in the developing endosperm of maize (Zea mays L.). The enzyme showed specificity for its substrates: lysine, α-ketoglutarate, and NADPH. Formation of the reaction product saccharopine was demonstrated. The pH optimum of the enzyme was close to 7, and the Km for lysine and α-ketoglutarate were 5.2 and 1.8 millimolar, respectively.This publication has 7 references indexed in Scilit:
- General and lysine specific control of saccharopine dehydrogenase levels in the yeast Saccharomycopsis lipolyticaBiochimie, 1979
- Lysine Catabolism in Barley (Hordeum vulgare L.)Plant Physiology, 1976
- Incorporation of leucine-14C and lysine-14C into protein in the developing endosperm of nomal and opaque-2 cornArchives of Biochemistry and Biophysics, 1970
- Saccharopine cleavage by a dehydrogenase of human liverBiochimica et Biophysica Acta (BBA) - Enzymology, 1970
- Conversion of lysine to saccharopine by human tissuesBiochimica et Biophysica Acta (BBA) - General Subjects, 1968
- A mixed layer for separation of amino acids by thin-layer chromatographyJournal of Chromatography A, 1966
- SACCHAROPINE AN INTERMEDIATE OF AMINOADIPIC ACID PATHWAY OF LYSINE BIOSYNTHESIS .4. SACCHAROPINE DEHYDROGENASE1966