Abstract
Eleven strains of P. coronafaciens [syringae], 4 strains of P. coronafaciens pathovar zeae, 6 strains of P. coronafaciens ssp. atropurpurea, 2 strains of P. striafaciens and 1 Pseudomonas sp. were compared by physiological, biochemical, serological and pathological tests. All strains tested utilized sucrose, glucose, fructose, glycerol and caprate as sole C sources, hydrolyzed Tween 80, reduced litmus milk and grew at 0.degree. C. All strains were arginine dihydrolase and oxidase negative and all strains failed to hydrolyze starch, reduce nitrate or grow at 41.degree. C. Cells of all strains were immunofluorescent positive when stained by the indirect immunofluorescence test with antiserum to cells of P. coronafaciens or P. coronafaciens ssp. atropurpurea. No differences among strains were detected in test tube agglutination tests. No significant differences were detected in the susceptibility of Avena sativa (oats), Secale cereale (rye), Triticum aestivum (wheat), Hordeum vulgare (barley), Bromus inermis (smooth bromegrass), B. japonicus (Japanese brome), B. secalinus (chess brome), B. tectorum (cheatgrass), Agropyron repens (quackgrass), Zea mays (maize) or Phleum pratense (timothy) to the different strains. All strains produced toxin in Wooley medium except P. striafaciens and strains of P. coronafaciens ssp. atropurpurea from Italian ryegrass. The present division of strains of P. coronafaciens based on minor differences in pathogenicity and symptomology is untenable.