Synthesis of hyaluronate in differentiated teratocarcinoma cells. Mechanism of chain growth
- 1 April 1983
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 211 (1) , 191-198
- https://doi.org/10.1042/bj2110191
Abstract
Hyaluronate could be labeled in vivo with [32P]phosphate. [32P]UDP in an .alpha.-glycosidic linkage constituted the reducing end of membrane-bound hyaluronate. The UDP is liberated during further chain elongation, indicating that chain growth occurs at the reducing end. [3H]Uridine could be incorporated into hyaluronate during synthesis on the isolated membraneous fraction from [3H]UDP-GlcNAc [N-acetyl glucosamine] and [3H]UDP-GlcA [glucosamine], confirming the identification of UDP as a constituent of membrane-bound hyaluronate. These results led to a model of hyaluronate chain elongation at the reducing end by alternate addition of the chains to the substrates. Membrane-bound pyrophosphatases or 5''-nucleotidase are suggested as modulators of hyaluronate synthesis.This publication has 43 references indexed in Scilit:
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