Effect of 5‘-deoxy-5’-isobutylthioadenosine on putrescine uptake and polyamine biosynthesis by chick embryo fibroblasts
- 15 June 1982
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 204 (3) , 853-859
- https://doi.org/10.1042/bj2040853
Abstract
The effect of 5''-deoxy-5''-S-isobutylthioadenosine (SIBA) on polyamine biosynthesis was studied by using cultured chick embryo fibroblasts. The drug inhibits the uptake of [14C]putrescine and its conversion into labeled spermidine or spermine. The inhibitory effect is reversed by removing the inhibitor after exposing the cells to the drug for 24 h. SIBA also caused a significant decrease in cellular spermine levels and an accumulation of putrescine. These changes are reversed by removing the inhibitor. SIBA had the same effect on chick embryo fibroblasts transformed by Rous sarcoma virus; a decrease in cellular spermine levels in SIBA-treated cells was observed. In all the experiments SIBA caused a reduction in the spermine/putrescine and spermidine/putrescine ratios. SIBA an inhibitor of transmethylation and it also interferes with polyamine biosynthesis, probably by blocking aminopropyltransferase.This publication has 30 references indexed in Scilit:
- Effect of a methyl-transferase inhibitor, 5′-deoxy-5′-s-isobutyl-thioadenosine (SIBA) on cAMP level and progesterone induced meiosis reinitiation in xenopus laevis oocytesBiochemical and Biophysical Research Communications, 1981
- DNA methylation and control of gene expressionNature, 1981
- Inhibition of nucleoside and sugar transport into cells by an oncostatic methylase inhibitor, 5′‐deoxy‐5′‐S‐isobutyl‐thioadenosine (SIBA)FEBS Letters, 1979
- Superinduction of ornithine decarboxylase by Actinomycin D and CordycepinBiochemical and Biophysical Research Communications, 1978
- Polyamines in rapid growth and cancerBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1978
- Ribosome binding to reovirus mRNA in protein synthesis requires 5′ terminal 7-methylguanosineCell, 1975
- Inhibition of virus-induced cell transformation by synthetic analogues of S-adenosyl homocysteineBiochemical and Biophysical Research Communications, 1975
- Polyamines and tumor cells: Effect of transformation of chick embryo fibroblasts by rous sarcoma virus on polyamine levelsBiochemical and Biophysical Research Communications, 1973
- Synthèses d'inhibiteurs des méthyl-transférases : analogues de la S-adénosyl homocystéineBiochimie, 1971
- Use of the Dansyl Reaction in Biochemical AnalysisPublished by Wiley ,1970