CYTOCHEMICAL STUDY ON THE LOCALIZATION OF ACID-PHOSPHATASE IN SACCHAROMYCES CEREVISIAE YEAST
- 1 January 1979
- journal article
- research article
- Vol. 21 (12) , 1403-+
Abstract
Optimal conditions of the cytochemical assay for acid phosphatase in protoplasts and whole cells of S. cerevisiae were described. Dimethylsulfoxide was used to increase the permeability of the yeast cell envelope. In the yeast cells, grown up to the end of the exponential phase, acid phosphatase is located mainly in the central vacuole and on the cell envelope surface. A considerable activity of acid phosphatase is demonstrable on the surface of the plasma membrane and within adjacent vesicles that represent, presumably, part of the endoplasmic reticulum. Acid phosphatase can be considered as a marker enzyme for yeast cell vacuoles.This publication has 2 references indexed in Scilit:
- Oxidative phosphorylation in yeast I. Isolation and properties of phosphorylating mitochondria from stationary phase cellsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1968
- [10] Assay of inorganic phosphate, total phosphate and phosphatasesPublished by Elsevier ,1966