Host Responses to Infectious Bovine Rhinotracheitis Virus

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Abstract
An investigation was made to determine if the nylon wool column technique of Julius et al., (9), could be used to fractionate bovine peripheral blood lymphocytes into T enriched (effluent) and B enriched (adherent) cells. After column filtration, the subpopulations were checked for surface-labeling characteristics with fluorescein-labeled anti-Ig serum (membrane fluorescence), phagocytic activity, response to mitogens, and their reactivity in two in vitro assays that quantitate cell-mediated immunity (CMI) against a bovine herpes virus-infectious bovine rhinotracheitis (IBR) virus. The effluent cells appeared enriched in T cells since few cells showed membrane fluorescence and these cells responded well to the T cell mitogens phytohemagglutinin (PHA) and concanavalin A (Con A). The adherent cells appeared enriched in B cells since many cells showed membrane fluorescence and the cells responded well to lipopolysaccharide (a B cell mitogen) but poorly to PHA and Con A. In the in vitro CMI assays against IBR virus, the effluent cells were far more reactive than the adherent cells implying that these assays are essentially measures of T cell activity. The implications of the results for the understanding of the mechanism of resistance to herpes virus infections is discussed.

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