Growth and Pathogenicity of Alfalfa Strain ofVerticillium albo-atrum

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Abstract
Thirty-eight isolates of V. albo-atrum form alfalfa (M. sativa) from diverse locations in the northwestern USA and western Canada (Britis Columbia) appeared the same on prune lactose yeast agar. Eight isolates representative of this area were alike in growth response to temperature on prune lactose yeast agar; ability to induce symptoms in alfalfa cultivars ''Vertus'' (moderately resistant to V. albo-atrum) and ''Apalachee'' (susceptible to V. albo-atrum); pathogenicity to certain cultivars in eggplant (Solanum melongena) cantaloupe (Cucumis melo), and watermelon (Citrulls lanatus); and not inducing symptoms in potato (S. tuberosum) or tomato (Lycopoersicon esculentum). The optimum temperature for growth in media was at or near 25.degree. C. Growth of an isolate from alfala of V. albo-atrum on osmotically adjusted media was greater at 27 and 30.degree. C than it was on the unamended agar medium. Only 1 alfalfa strain of V. albo-atrum exists in the area and it may have the potential to become more widespread in the USA than strains of V. albo-atrum isolated from other crops.

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