GENETIC ANALYSIS OF H‐2 LINKED GENE(S) AFFECTING EARLY MOUSE EMBRYO DEVELOPMENT

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Abstract
The number of cells in preimplantation mouse embryos of different H-2 haplotypes was analyzed. Embryos of the H-2k haplotype have fewer cells per embryo than those of the H-2b haplotype. By analyzing reciprocal congenic pairs of mice it was demonstrated unequivocally that slow development is linked to the H-22 haplotype and fast development to the H-2k haplotype. The gene(s) in the H-2 complex which influence early mouse embryo development were named Ped: preimplantation embryo development. Analysis of F1 hybrid embryos showed that fast development is dominant. Reciprocal F1 crosses yielded identical results, which indicated there was no apparent effect of the maternal egg cytoplasm on Ped gene expression. Analysis of F2 and backcross embryos was consistent with the interpretation that there is a major gene located in the H-2 complex (Ped), which is modified by environment and genetic background, that influences early mouse embryo development.