RNA editing of rapeseed mitochondrial atp9 transcripts. RNA editing changes four amino acids, but termination codon is already encoded by genomic sequence.
- 1 January 1993
- journal article
- Published by Genetics Society of Japan in The Japanese Journal of Genetics
- Vol. 68 (1) , 47-54
- https://doi.org/10.1266/jjg.68.47
Abstract
The gene encoding subunit 9 of Fo-ATPase of rapeseed mitochondria has been isolated. The complete genomic DNA sequence and cDNA sequence corresponding to the atp9 gene transcript have been determined by a method involving cDNA synthesis, using specific oligonucleotides as primers, followed by PCR amplification, cloning and sequencing of the amplification products. In comparison of cDNA sequences to genomic one, four modifications, C-to-U conversions, have been found. When compared with RNA editing patterns of atp9 transcripts among plant mitochondria, that of rapeseed atp9 transcript is more simple; there are only four editing sites on the coding region, and its termination codon is already encoded by genomic sequence.Keywords
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