Meristem transformation of sunflower via Agrobacterium
- 1 July 1990
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 9 (2) , 55-60
- https://doi.org/10.1007/bf00231548
Abstract
For transformation of sunflower (Helianthus annuus L. cv. Zebulon), shoot apical meristems were dissected from seeds and cocultivated with a disarmed Agrobacterium tumefaciens strain harboring a binary vector carrying genes encoding GUS- and NPTII-activity. The influence of the media conditions, the time of cocultivation and the stage of the developing seed on shoot development and meristem transformation was analysed. Transformants were selected by their ability to grow on kanamycin. Transformation was confirmed by assays for GUS and NPTII. GUS-positive shoots were rooted on rockwool and transferred to soil. Transformation of shoot meristem cells occurred at low frequencies. Chimaeric expression of the two genes was observed in transformed plants. Integration of the foreign DNA in the sunflower genome was confirmed with the polymerase chain reaction.Keywords
This publication has 11 references indexed in Scilit:
- Construction of an intron-containing marker gene: Splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformationMolecular Genetics and Genomics, 1990
- Genetically Engineering Plants for Crop ImprovementScience, 1989
- A small-scale procedure for the rapid isolation of plant RNAsNucleic Acids Research, 1989
- Cell lineage patterns in the shoot meristem of the sunflower embryo in the dry seedDevelopmental Biology, 1989
- Dot assay for neomycin phosphotransferase activity in crude cell extractsAnalytical Biochemistry, 1987
- The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNAJournal of Bacteriology, 1986
- Isolation, culture and callus regeneration of protoplasts of wild and cultivated Helianthus speciesPlant Cell Reports, 1986
- A new sensitive method for qualitative and quantitative assay of neomycin phosphotransferase in crude cell extractsGene, 1984
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962