Functional expression of gastric H,K‐ATPase using the baculovirus expression system
- 30 August 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 329 (3) , 277-282
- https://doi.org/10.1016/0014-5793(93)80237-o
Abstract
A novel approach to construct a single recombinant baculovirus expressing two protein subunits simultaneously by replacing polyhedrin as well as p10 coding sequences is described. The recombinant baculovirus expressed the α‐ as well as the β‐subunit of the gastric H.K‐ATPase. Sf9 cells infected with this virus exhibited a K+‐ and SCH 28080‐sensitive ATP‐dependent phosphorylation capacity in purified Sf9 membranes similar to Natlve H,K‐ATPase. This activity was not present in control membranes containing only one of the two H,K‐ATPase subunits. We therefore conclude that both subunits are essential for the phosphorylation capacity of H.K‐ATPase.Keywords
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