Plasma disappearance of rabbit α2 HS-glycoprotein and its uptake by bone tissue

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Abstract
Plasmaα2HS-glycoprotein is specifically accumulated in calcified tissues. In the present studies this glycoprotein was isolated from plasma and after iodination with iodine-125 was injected intravenously into young rabbits. The tissue distribution and plasma disappearance rate of this radioactively labeled material were determined. Of the various tissues studied, bone showed the greatest retention of labeled glycoprotein expressed as percentage of the injected dose per gram tissue relative to the plasma content. The rate of loss of iodinatedα2HS-glycoprotein from plasma was similar to that ofα2HS-glycoprotein labeled endogenously by using14C-glucosamine or3H-glucosamine. The uptake of exogenously labeled3I-α2HS-glycoprotein into bone tissue expressed as a percentage of the injected dose was similar to that of endogenously labeled14C-α2HS-glycoprotein. These results suggest that the125I-labeled material can be used to study further the metabolism ofα2HS-glycoprotein by bone tissue.