The mechanism of K cell (antibody-dependent) cell mediated cytotoxicity I. The release of different cell components

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Abstract
The release of different cell components during K cell (antibody-dependent) cytotoxicity of P815 mastocytoma cells by rat spleen cells has been studied. The release of the small molecules chromium-51 and phosphoryl choline follow the same time course as the release of macromolecular protein and RNA, suggesting a sudden rupture of the cytoplasmic membrane. These components are rapidly released over the first 15–30 min whereas in T cell cytotoxicity the rate of release is approximately linear with time (Sanderson 1976 a ). This suggests that cell death occurs more rapidly after K cell than after T cell contact. Nicotinamide and rubidium-86 are released more rapidly than these other components but release does not continue after 15–30 min. The release of these two components may be mainly a result of K cell contact and some K cell contacts may not progress to target cell death. The release of DNA shows a 30–45 min lag suggesting that the nuclear rupture is secondary to cytoplasmic rupture.