The Use of Iodinated RNA for Gene Localization
- 1 June 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (6) , 1860-1864
- https://doi.org/10.1073/pnas.70.6.1860
Abstract
Carrier-free iodination of 5S RNA with (125)I yields a probe suitable for use in cytological RNA-DNA hybridization studies. The genes coding for 5S ribosomal RNA in Drosophila could be localized by autoradiography after a 2-day exposure, whereas a 2-month exposure is needed when the best available [(3)H]RNA is used. The procedure introduces one covalently bound (125)I atom per 100 nucleotides, resulting in a specific activity of over 10(8) dpm/mug of RNA. The method may be readily applied to other problems involving molecular hybridization techniques.Keywords
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