Biosynthesis of diaminopimelic acid and lysine in Escherichia coli. 1. The incorporation of 14C from various organic precursors into the diaminopimelic acid of a lysine-requiring mutant

Publisher Website
Google Scholar
Abstract
Escherichia coli 26-26 was grown on a suboptimum concentration of lysine with various C14-labelled compounds to determine which materials could act as carbon-chain precursors for diaminopimelic acid. [C14]Pyruvate and [C14]aspartate were incorporated into the intracellular diaminopimelic acid but not into the diaminopimelic acid which accumulated in the culture filtrate. [C14]Glucose and [2-C14]acetate were incorporated into the diaminopimelic acid both in the culture filtrate and in the cells. A mixture of the meso- and LL-isomers of [C14]diaminopimelic acid was isolated from the culture filtrate of the lysine-requiring mutant E. coli 26-26 grown with [C14]glucose as main C source.