Erythrosin 5′‐isothiocyanate labels Cys549 as part of the low‐affinity ATP binding site of Na+/K+‐ATPase1
- 11 December 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 441 (1) , 103-105
- https://doi.org/10.1016/s0014-5793(98)01533-6
Abstract
The high-affinity E1ATP site of Na+/K+-ATPase labeled with fluorescein 5′-isothiocyanate and its E2ATP site labeled with erythrosin 5′-isothiocyanate (ErITC), as was shown recently [Linnertz et al. (1998) J. Biol. Chem. 273, 28813–28821], reside on separate and adjacent catalytic α subunits. This paper provides evidence that specific labeling of the E2ATP binding site with ErITC resulted in a modification of the Cys549 residue in the tryptic fragment with the sequence Val545-Leu-Gly-Phe-Cys549-His550. Hence, Cys549 is part of or close to the low-affinity E2ATP binding site of Na+/K+-ATPase.Keywords
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