Increased collagen synthesis in skin fibroblasts from patients with primary hypertrophic osteoarthropathy. evidence for trans–activational regulation of collagen transcription
- 1 August 1994
- journal article
- research article
- Published by Wiley in Arthritis & Rheumatism
- Vol. 37 (9) , 1386-1394
- https://doi.org/10.1002/art.1780370918
Abstract
Objective. To investigate collagen synthesis in skin fibroblasts from patients with primary hypertrophic osteoarthropathy (HOA), a disorder characterized clinically by skin thickening.Methods. Collagenase–digestible protein, messenger RNA (mRNA) levels, and transcriptional activity of the α1(I) procollagen gene were assessed in skin–derived fibroblast lines.Results. Compared with fibroblasts from uninvolved skin, fibroblasts from involved skin had elevated levels of collagen synthesis and α1(I) procollagen mRNA, and increased transcriptional activity of the α1(I) procollagen promoter.Conclusion. Abnormalities of collagen synthesis in fibroblasts from patients with primary HOA can be accounted for, at least in part, by a trans–activated upregulation of collagen transcription.Keywords
This publication has 35 references indexed in Scilit:
- The Clinical Spectrum of Pachydermoperiostosis (Primary Hypertrophic Osteoarthropathy)Medicine, 1991
- Comparative Effects of Interleukin-1 and Tumor Necrosis Factor-α on Collagen Production and Corresponding Procollagen mRNA Levels in Human Dermal FibroblastsJournal of Investigative Dermatology, 1991
- Heterogeneity in hormone responses and patterns of collagen synthesis in cloned dermal fibroblasts.Journal of Clinical Investigation, 1990
- Pachydermoperiostosis (primary hypertrophic osteoarthropathy): report of a case with evidence of endothelial and connective tissue involvement.Annals of the Rheumatic Diseases, 1989
- Inhibition of excessive scleroderma fibroblast collagen production by recombinant γ‐interferon: Association with a coordinate decrease in types I and III procollagen messenger RNA levelsArthritis & Rheumatism, 1986
- Stimulation of normal human fibroblast collagen production and processing by transforming growth factor-βBiochemical and Biophysical Research Communications, 1986
- Fibroblast prostaglandin E2 synthesis. Persistence of an abnormal phenotype after short-term exposure to mononuclear cell products.Journal of Clinical Investigation, 1983
- Mononuclear cell modulation of connective tissue function: suppression of fibroblast growth by stimulation of endogenous prostaglandin production.Journal of Clinical Investigation, 1980
- Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donorCell, 1978
- Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteinsBiochemistry, 1971