Nucleotide sequence of F 0 -ATPase proteolipid (subunit 9) gene of maize mitochondria
- 1 February 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (4) , 1015-1019
- https://doi.org/10.1073/pnas.82.4.1015
Abstract
The FO-ATPase proteolipid, also referred to as subunit 9 or the dicyclohexylcarbodiimide-binding protein, is encoded by a mitochondrial gene in maize designated atp 9. The clone containing atp 9 was selected for investigation from a mitochondrial DNA library because of its abundant transcript in total maize mitochondrial RNA preparations. Sequence anslysis of the clone revealed an open reading frame that was readily identified by its nucleotide homology with the ATPase subunit 9 gene of yeast. As deduced from the nucleotide sequence, the maize ATPase subunit 9 protein contains 74 amino acids with a MW of 7368. Substantial amino acid sequence homology is conserved among maize, yeast, bovine and Neurospora mitochondrial ATPase subunit 9 proteins, regardless of whether the gene is nuclearly encoded (bovine and Neurospora) or mitochondrially encoded (yeast and maize). RNA transfer blot analysis indicated that the gene sequence is actively transcribed, producing an initial transcript that is large and extensively processed.This publication has 20 references indexed in Scilit:
- Pronounced structural similarities between the small subunit ribosomal RNA genes of wheat mitochondria and Escherichia coli.Proceedings of the National Academy of Sciences, 1984
- The plant mitochondrial genome and its mutantsCell, 1983
- Partial Sequence Analysis of the 5S to 18S rRNA Gene Region of the Maize Mitochondrial GenomePlant Physiology, 1983
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- The making of strand-specific M13 probesGene, 1982
- The zea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codonsCell, 1981
- Transcription maps of mtdnas of two strains of saccharomyces: transcription of strain-specific insertions; complex rna maturation and splicingCell, 1979
- The Dicyclohexylcarbodiimide‐Binding Protein of the Mitochondrial ATPase Complex from Neurospora crassa and Saccharomyces cerevisiaeEuropean Journal of Biochemistry, 1979
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977