STUDIES ON CRYSTAL-INDUCED CHEMOTACTIC FACTOR .2. ROLE OF PHAGOCYTOSIS

  • 1 January 1975
    • journal article
    • research article
    • Vol. 85  (4) , 631-636
Abstract
The interaction of polymorphonuclear leukocytes (PMN''s) and urate crystals leads to the formation of a chemotactic factor. When PMN''s [rabbit] were incubated with cytochalasin B, an inhibitor of phagocytosis, and urate crystals, no chemotactic activity appeared in the lysosomal extract of the cells. Likewise, no chemotactic activity was present in the media of PMN''s incubated with crystals although lysosomal enzyme activity was increased. Phagocytosis apparently is required for chemotactic activity to appear. Cytochalasin B increased the release of lysosomal enzyme B-glucuronidase but not of cytoplasmic enzyme lactic dehydrogenase from PMN''s when the cell encountered urate crystals.

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