Electron microscopy study of Qβ replicase
- 15 February 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 228 (2) , 263-267
- https://doi.org/10.1016/0014-5793(88)80012-7
Abstract
Purified preparations of Qβ replicase have been studied by electron microscopy using a negative staining technique, and a three-dimensional model of the enzyme molecule has been constructed. The molecule of this four-subunit protein appears to be a compact structure having a size of 100 ± 10 Å; it is subdivided into two unequal bipartite subparticles. The conclusion has been made that all the constituent subunits, including the ribosomal protein S1, acquire a globular conformation when associated in the replicase complexKeywords
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