DEPLETION AND REPLENISHMENT OF CELLULAR CYCLIC ADENOSINE-MONOPHOSPHATE IN HEMORRHAGIC-SHOCK

  • 1 January 1977
    • journal article
    • research article
    • Vol. 145  (6) , 877-881
Abstract
Hemorrhagic shock in rats was produced by bleeding them to a mean arterial blood pressure of 40 mmHg, which was maintained for 2 h. After sacrifice small pieces of liver, kidney, muscle and brain were quickly removed and frozen in liquid N. A protein-free extract of tissues was prepared, and cyclic [c]AMP was measured by the radioimmunoassay procedure. Analysis of liver, kidney, muscle and brain showed that there was a significant reduction in cAMP and ATP levels. The decrease in cAMP levels follows the same trend in the various organs as the decrease in ATP levels, suggesting that these 2 events are related. Infusion of ATP magnesium chloride, 25 .mu.mol each, at the end of the shock period restored the cellular cAMP and ATP levels. The precise mechanism for depletion and replenishment of cAMP levels during shock is not known.