cAMP‐dependent activation of protein synthesis correlates with dephosphorylation of elongation factor 2
- 15 February 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 228 (2) , 327-331
- https://doi.org/10.1016/0014-5793(88)80025-5
Abstract
The addition of 5 mM cAMP to a cell-free translation system from rabbit reticulocytes increases the rate of protein synthesis 3–5-fold. Lower concentrations of cAMP (0.005, 0.05 and 0.5 mM) have no effect on translation in this system. cAMP at all the concentrations tested stimulates the phosphorylation of the same pattern of polypeptides, while 5 mM cAMP additionally stimulates dephosphorylation of the 95 kDa polypeptide identified as elongation factor 2 (EF-2). Testing of the preparations of EF-2 with a different content of the phosphorylated form in poly(U)-directed poly(Phe) synthesis reveals that the EF-2 activity correlates with the fraction of non-phosphorylated EF-2. Thus cAMP-dependent activation of protein synthesis seems to be due to dephosphorylation of EF-2.Keywords
This publication has 19 references indexed in Scilit:
- Ca2+/calmodulin‐dependent phosphorylation of elongation factor 2FEBS Letters, 1987
- Regulation of Nerve Growth Factor Action on Nsp100 Phosphorylation in PC12h Cells by CalciumJournal of Neurochemistry, 1986
- Phosphorylation of elongation factor 1 in polyribosome fraction of rabbit reticulocytesFEBS Letters, 1984
- Endogenous ADP‐ribosylation of elongation factor 2 in polyribosome fraction of rabbit reticulocytesFEBS Letters, 1984
- The control of protein synthesis during heat shock in Drosophila cells involves altered polypeptide elongation ratesCell, 1983
- Phosphorylation of initiation factor eIF-2 and the control of reticulocyte protein synthesisCell, 1977
- Translational Control of Protein SynthesisAnnual Review of Biochemistry, 1976
- The regulation of protein synthesis in animal cells by serum factorsBiochemistry, 1976
- The effect of cyclic AMP and related compounds on the control of protein synthesis in reticulocyte lysatesBiochemical and Biophysical Research Communications, 1974
- Factors affecting the rate of protein synthesis in lysate systems from reticulocytesArchives of Biochemistry and Biophysics, 1968