Effects of an Active Substance Isolated from the Roots ofAngelica shkiokianaon Leukotriene and Monohydroxyeicosatetreaenoic Acid Biosyntheses in Human Polymorphonuclear Leukocytes

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Abstract
It was found that the EtOAc fraction of the roots of Angelica shkiokiana Makino inhibited calcium ionophore-induced LTB4 and LTC4 formations in human polymorphonuclear leukocytes (PMN-L). And the active substance isolated from this root of was elucidated to be 3′ (R), 4′ (R)-3′-epoxyangeloyloxy-4′-acetoxy-3′, 4′-dihydroseselin (YN-1). Furthermore, YN-I at concentrations of 4-400 µg/ml inhibited calcium ionophore-induced 12-HETE, 5-HETE, LTB4, and LTC4 formation in human PMN-L, dose-dependently, as shown by measurements with reversed phase HPLC and radioimmunoassay systems.