Non‐uniform distribution of phospholipids in (Na+ + K+‐ATPase‐rich membranes from Torpedo marmorata electric organ evidenced by spin—spin interactions between spin‐labeled phospholipids
- 14 November 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 163 (2) , 245-249
- https://doi.org/10.1016/0014-5793(83)80828-x
Abstract
(Na+ + K+)‐ATPase membranes from Torpedo marmorata electric organ were labeled with high concentrations of paramagnetic phospholipid analogs (up to 5 mol of spin labels for 100 mol of endogeneous phospholipids). The mobile lipid bilayer component of the complex resonance spectra obtained showed low‐field linewidth broadening due to spin—spin interactions; this was used as a measure of probe concentration in this compartment. Our results show that in the lipid shell surrounding the protein, there is a considerable enrichment in negatively charged phospholipids over neutral ones.Keywords
This publication has 18 references indexed in Scilit:
- Fractionation of protein components of plasma membranes from the electric organ of Torpedo marmorataBiochimica et Biophysica Acta (BBA) - Biomembranes, 1982
- Simulation of electron spin resonance spectra of spin-labeled fatty acids covalently attached to the boundary of an intrinsic membrane protein. A chemical exchange modelJournal of Magnetic Resonance (1969), 1982
- ESR spin-label studies of lipid-protein interactions in membranesBiophysical Journal, 1982
- Lateral diffusion of lipids in sarcoplasmic reticulum membranes is area limitedNature, 1981
- The fraction of phosphatidylinositol that activates the (Na+ + K+)-ATPase in rabbit kidney microsomes is closely associated with the enzyme proteinBiochimica et Biophysica Acta (BBA) - Biomembranes, 1981
- Spin-label studies of head-group specificity in the interaction of phospholipids with yeast cytochrome oxidaseBiochemistry, 1981
- Lipid-protein multiple binding equilibriums in membranesBiochemistry, 1981
- Boundary lipid in proteolipid—lipid recombinants revealed by fluorescence energy transfer and spin probe studyFEBS Letters, 1981
- Phosphatidylinositol as the endogenous activator of the (Na+ + K+)-ATPase in microsomes of rabbit kidneyBiochimica et Biophysica Acta (BBA) - Biomembranes, 1978
- Reaction of (sodium(1+)-potassium(1+) ion)-activated ATPase with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole: evidence for an essential tyrosine at the active siteBiochemistry, 1978