Electron microscopy of some rock phosphate dissolving bacteria and fungi

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Abstract
BacteriaPseudomonas striata, Bacillus polymyxa, B. megaterium andB. pulvifaciens, and fungiAspergillus awamori, A. niger andPenicillium digitatum dissolve tricalcium phosphate and, much less, Mussorie and Udaipur rock phosphate. The solubilizing power of fungi was higher than that of bacteria, the highest being withA. awamori andA. niger, and withP. striata. Electron microscopy of the various cultures showed an electron-dense layer on the bacterial surface after negative staining. The size of phosphate particles decreased by the microbial action, with tricalcium phosphate from 140 — 250 to 30 — 90 nm after three weeks of incubation.