Primary structure of human intrinsic factor: progress report on cyanogen bromide fragmentation
- 1 January 1978
- journal article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 38 (7) , 649-653
- https://doi.org/10.3109/00365517809102431
Abstract
Human intrinsic factor purified by labile ligand affinity chromatography was cleaved with cyanogen bromide and fractionated by gel filtration. Four of the fragments were purified and sequenced to a total of eighty-four amino acid residues. Including the N-terminal amino acids this amounts to one third of the total amino acid sequence of human intrinsic factor. One of the fragments contained a tyrosine labelled only on iodination of intrinsic factor devoid of cobalamin.Keywords
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