Total synthesis of the cystatin α gene and its expression in E. coli
Open Access
- 26 September 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 238 (1) , 116-118
- https://doi.org/10.1016/0014-5793(88)80238-2
Abstract
A gene encoding cystatin α has been chemically synthesized, cloned and expressed in E. coli. The gene of 318 base pairs was assembled by enzymatic ligation of 19 oligonucleotides and cloned into a pBR322‐derived expression plasmid down stream of the tac promoter. The expression product of the synthetic gene has been purified by Sephadex G‐50 column chromatography and shown to have the same properties as those of the authentic protein isolated from rat epidermis.Keywords
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