Feasibility of Screening for Penicillinase-Producing Neisseria gonorrhoeae from Primary Culture Plates by Using a Rapid Microacidometric Test

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Abstract
We have shown that it is possible to screen for penicillinase-producing Neisseria gonorrhoeae directly from primary culture plates. Experiments involving the cocultivation of four genera of beta-lactamase-positive (β-lac + ) bacteria and a beta-lactamase-negative (β-lac − ) N. gonorrhoeae on modified Thayer-Martin medium indicated that suppressed or inhibited β-lac + bacteria did not give false-positive results when isolated β-lac − colonies of gonococci were tested. Colonies were assayed for β-lac production by an adaptation of a microacidometric method in which bacteria could be tested before or after the addition of oxidase reagent.