Electrogenicity at the donor/acceptor sides of cyanobacterial photosystem I

1 December 1996

journal article
Published by Springer Nature in Journal of Bioenergetics and Biomembranes

Vol. 28 (6) , 517-522
https://doi.org/10.1007/bf02110441

Abstract

To study electrogenesis the photosystem I particles fromSynechococcus elongatus were incorporated into asolectin liposomes, and fast kinetics of laser flash-induced electric potential difference generation has been measured by a direct electrometric method in proteoliposomes adsorbed on a phospholipid-impregnated collodion film. The photoelectric response has been found to involve three electrogenic stages associated with (i) iron-sulfur center F_x reduction by the primary electron donor P700, (ii) electron transfer between iron-sulfur centers F_x and F_A/F_B, and (iii) reduction of photo-oxidized P700⁺ by reduced cytochromec ₅₅₃. The relative magnitudes of phases (ii) and (iii) comprised about 20% of phase (i).

Keywords

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