Phosphatidic Acid-Induced Calcium Mobilization in Osteoblasts1

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Abstract
Phosphatidic acid (PA) evoked a transient increase in the cytosolic free Ca2+ concentration ([Ca2+]1) in osteoblasts isolated from neonatal mouse calvaria. This increase was observed in both low (below 150 μM) and high (1.26 mM) Ca2+-containing medium. In contrast, other phospholipids, such as phosphatidylethanolamine, phosphatidylcholine, and phosphatidylinositol, failed to increase [Ca2+]1 in osteoblasts. In high Ca2+-containing medium, A23187 also increased [Ca2+]1 in the cells, but the mode of the change was different from that in the case of PA. These results suggest that PA may induce Ca2+-mediated cellular responses through Ca2+ release from intracellular stores in osteoblasts.