High performance liquid chromatographic determination of ketoconazole in human serum
- 1 December 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Antimicrobial Chemotherapy
- Vol. 18 (6) , 757-763
- https://doi.org/10.1093/jac/18.6.757
Abstract
A reversed-phase high-performance liquid chromatographic (HPLC) method with terconazole as internal standard is described for the rapid analysis of ketoconazole in human serum. Except for diazepam no other drug interfered with the analysis. The lowest reproducible limit of the method was 0.05 mg/l. The coefficient of variation of the procedure was ≤7.5% over a range of ketoconazole concentrations from 1.0 to 10.0 mg/l and ≤16.75% over a range from 0.05 to 0.5 mg/l. Comparison with a microbiological method showed good correlation.Keywords
This publication has 1 reference indexed in Scilit:
- Impairing effect of food on ketoconazole absorptionAntimicrobial Agents and Chemotherapy, 1982