DETECTION OF CREATINE-KINASE ISOENZYME CK1 IN SERUM
- 1 January 1979
- journal article
- research article
- Vol. 9 (1) , 80-83
Abstract
The brain isoenzyme of creatine kinase (CK) (EC 2.7.3.2) was identified in the serum of 21 of 950 patients tested. Samples were received over 20 mo. Analysis was performed by electrophoresis on agarose gel with isoenzyme bands visualized by fluorescence after enzyme coupled reactions producing NADH. Specialized substrate preparations were used to rule out the possibility that observed fluorescence was due to enzymatic activity other than that of CK isoenzymes. Patients who were hypothermic and/or had undergone cardiopulmonary resuscitation accounted for 19 of 21 patients showing the brain isoenzyme. The inciting mechanism for the release of CK1 into the circulation is not clearly understood.This publication has 3 references indexed in Scilit:
- Brain-Type Creatine Kinase IsoenzymeArchives of Neurology, 1977
- Detection of creatine kinase BB isoenzyme in sera of patients undergoing aortocoronary bypass surgeryClinica Chimica Acta; International Journal of Clinical Chemistry, 1977
- Serum creatine kinase isoenzyme pattern in nervous system atrophies and neuromuscular disordersClinica Chimica Acta; International Journal of Clinical Chemistry, 1976