In Vitro Culture of Orobanche aegyptiaca

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Abstract
Calli derived from seedlings of the parasitic plant Orobanche aegyptiaca were grown on enriched nutrient agar. Growth was enhanced by the addition of 0.1 mg 1−1 NAA and 5 % coconut milk. The calli developed two kinds of elongated protrusions: thin ones which resemble the young root radicle and thick ones which resemble the adventitious roots of Orobanche plants. Both formed pad-like structures when touching the substrate. In liquid culture, some of the calli were observed to surround isolated cultured tomato roots.

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