Demonstration of calcium in dermal melanocytes of Xenopus laevis and Poecilia reticulata with electron energy‐loss spectroscopy and electron spectroscopic imaging

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Abstract
The subcellular distribution of calcium in dermal melanocytes of Xenopus laevis and Poecilia reticulata has been analysed. Using two cytochemical methods, phosphate precipitation and a combined oxalate‐pyroantimonate technique, electron energy‐loss spectroscopy and electron spectroscopic imaging have been applied for elemental analysis. Both precipitation techniques revealed a high calcium content in the melanosomes of both species. Calcium was also located in the vicinity of collagen fibrils and in the plasma membrane.