Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.
- 1 August 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (8) , 4887-4891
- https://doi.org/10.1073/pnas.78.8.4887
Abstract
The complete 7410 nucleotide sequence of the poliovirus type I genome was obtained from cloned c[complementary]DNA. Double-stranded poliovirus cDNA was synthesized and inserted into the Pst I site of plasmid pBR322 and 3 clones were derived that together provided DNA copies of the entire poliovirus genome. Two of the clones contained inserts of 2.5 and 6.5 kbases and represented all but the 5'' 115 bases of poliovirus RNA. A 3rd clone was generated from primer-extended DNA and contained sequences from the 5'' end of the viral RNA. An open reading frame that was identified in the nucleotide sequence starting 743 bases from the 5'' end of the RNA and extending to a termination codon 71 bases from the 3'' end contained known poliovirus polypeptide sequence.Keywords
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