Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract
Open Access
- 25 August 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (16) , 3907-3918
- https://doi.org/10.1093/nar/9.16.3907
Abstract
We describe the preparation of a cell-free extract from Drosophila Kc cells which allows transcription of a variety of cloned eukaryotic RNA polymerase III genes. The extract has low RNA-processing nuclease activity and thus the major products obtained are primary transcripts.Keywords
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