Abstract
Spectrophotometric determinations furnish a simple means of depicting certain characteristics of the blood, and the curves show the presence (or absence) of hematin, reduced hemoglobin, oxyhemoglobin, and possibly methemoglobin in peripheral or surface tissues and blood. By further analysis of spectrophotometric data into the fundamental red, green, and violet excitation values, it is possible to differentiate between changes or variations in skin color due to pigment or characteristics dependent on amount, quality, and distribution of the peripheral blood supply. The method is of value in studying various pathological conditions. Analyses may be made in terms of the 3 attributes of color, or relative luminosity (brilliance), dominant wave length (hue), and purity (saturation).

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