Indispensable role of the transcription factor PEBP2/CBF in angiogenic activity of a murine endothelial cell MSS31
- 6 January 2000
- journal article
- Published by Springer Nature in Oncogene
- Vol. 19 (1) , 106-114
- https://doi.org/10.1038/sj.onc.1203257
Abstract
Mice lacking the AML1/PEBP2alphaB/CBFa2 gene or PEBP2beta/CBFb gene exhibit a defect in definitive hematopoiesis and die in utero because of hemorrhage in the central nervous system. Hematopoiesis in the embryo is considered to be tightly associated with vascular development. Here we examined whether PEBP2/CBF plays any role in angiogenesis besides that in definitive hematopoiesis. We found that AML1/PEBP2alphaB/CBFa2, PEBP2alphaA/CBFa1, and PEBP2beta/CBFb were expressed in a murine endothelial cell line MSS31. The expression of these molecules as well as the DNA binding activity of PEBP2/CBF were augmented by angiogenic growth factors such as bFGF and VEGF. Moreover, the expression of PEBP2 alpha/CBFa protein in endothelial cells was confirmed at the site of angiogenesis in vivo. To further clarify the role of PEBP2/CBF in angiogenesis, we established permanent transfectants of PEBP2 beta-MYH11 gene, one that interacts with the runt domain of the alpha subunit and deregulates PEBP2/CBF in a dominant interfering manner. Proliferation, migration, and tube formation of the PEBP2 beta-MYH11 transfectants were significantly reduced in comparison with those activities of the mock transfectants. These results suggest that transcription factor PEBP2/CBF plays an important role in angiogenesis.Keywords
This publication has 45 references indexed in Scilit:
- CBFβ-SMMHC, expressed in M4Eo AML, reduced CBF DNA-binding and inhibited the G1 to S cell cycle transition at the restriction point in myeloid and lymphoid cellsOncogene, 1997
- Targeted Disruption of Cbfa1 Results in a Complete Lack of Bone Formation owing to Maturational Arrest of OsteoblastsPublished by Elsevier ,1997
- Differentiation dependent expression and distinct subcellular localization of the protooncogene product, PEBP2β/CBFβ, in muscle developmentOncogene, 1997
- Ets-1 regulates angiogenesis by inducing the expression of urokinase-type plasminogen activator and matrix metalloproteinase-1 and the migration of vascular endothelial cellsJournal of Cellular Physiology, 1996
- Isolation of Angiopoietin-1, a Ligand for the TIE2 Receptor, by Secretion-Trap Expression CloningCell, 1996
- Failure of Embryonic Hematopoiesis andLethal Hemorrhages in Mouse Embryos Heterozygousfor a Knocked-In Leukemia Gene CBFB–MYH11Cell, 1996
- Lymphoid Potential, Probed before Circulation in Mouse, Is Restricted to Caudal Intraembryonic SplanchnopleuraCell, 1996
- Dominant-negative and targeted null mutations in the endothelial receptor tyrosine kinase, tek, reveal a critical role in vasculogenesis of the embryo.Genes & Development, 1994
- Detection of fusion transcripts generated by the inversion 16 chromosome in acute myelogenous leukemiaBlood, 1994
- Fusion Between Transcription Factor CBFβ/PEBP2β and a Myosin Heavy Chain in Acute Myeloid LeukemiaScience, 1993