Characterization of the transcript for a depressant insect selective neurotoxin gene with an isolated cDNA clone from the scorpionButhotus judaicus
- 20 August 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 269 (1) , 229-232
- https://doi.org/10.1016/0014-5793(90)81161-g
Abstract
The poly(A)+ mRNA isolated from the venomous terminal segments of the scorpion Buthotus judaicus was reversed transcribed into cDNA. PCR amplification of the cDNA in presence of oligonucleotide primers prepared on basis of the known amino acid sequence of the depressant insect toxin II yielded a 125 bp long product. This fragment was cloned and its sequence determined. The deduced amino acid sequence has revealed a complete homology with the amino acid sequence of the toxin. This clone was used to probe a Northern blot resolving the poly(A)+ and poly(A)− fractions derived from the scorpion. An organ specific 360 nucleotide transcript which might be the processed product of a 4.0 kb precursor was elucidated. This cDNA clone may pave the way for a molecular genetic approach to study the structure-function relationship of scorpion selective insect toxinsKeywords
This publication has 6 references indexed in Scilit:
- Use of the DNA polymerase chain reaction for homology probing: isolation of partial cDNA or genomic clones encoding the iron-sulfur protein of succinate dehydrogenase from several species.Proceedings of the National Academy of Sciences, 1989
- An excitatory and a depressant insect toxin from scorpion venom both affect sodium conductance and possess a common binding siteArchives of Biochemistry and Biophysics, 1985
- Detection, Purification and Receptor Binding Assays of Insect Selective Neurotoxins Derived from Scorpion VenomsPublished by Springer Nature ,1985
- The nucleotide sequence of the rat cytoplasmic β–actin geneNucleic Acids Research, 1983
- pEMBL: a new family of single stranded plasmidsNucleic Acids Research, 1983
- Purification, characterization and action of two insect toxins from the venom of the scorpion buthotus judaicusBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1982