Effect of Dimethyl Sulfoxide and Glycerol on Cation Content of Freeze-Thawed Cardiac Muscle

Abstract
The effects of dimethyl sulfoxide (DMS) and glycerol on K and Na content of freeze-thawed isolated rabbit heart muscle were determined. Both right atrial and left ventricular tissue lost K and gained Na after freeze-thawing compared to freshly dissected tissue. The presence of 10% DMS or 15% glycerol during freeze-thawing did not prevent this loss of K or gain of Na. Glycerol also produced a loss of tissue K even in the absence of freeze- thawing.

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