Immunochemical detection of sequence-specific modifications to DNA induced by UV light

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Abstract
Sequence specificity of antibodies to UV-damaged DNA has not been described previously. The antisera Investigated here were specific for UV-modifled DNA and were absolutely dependent upon the presence of thymine residues. Using a series of oligonucleotides in competition ELISA, Increased Inhibition was observed with Increasing chain length of UV-polythymldylate. A minimum of three adja cent thymlnes was required for effective inhibition; alone, dimers of thymine were poor antigens. Although UV Irradiated poly(dC) was not anfigenic, cytosines could partially replace thymines within the smallest effective epitope (T-T-T) with a high degree of sequence specificity, not previously described. The main epitope induced by UV was formed from adjacent thymines and either a 3′ or a 5′ pyrhnidlne.

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