TRANSFER OF GLUCOSE-1-PHOSPHATE FROM UDP-GLUCOSE TO LIPID ACCEPTORS IN PLANTS

  • 1 January 1976
    • journal article
    • research article
    • Vol. 26  (5) , 364-370
Abstract
Particulated preparations from pea seedlings formed lipid-linked glucose from UDP(14C)-glucose and endogenous acceptors at 0.degree. C. The product was identified as a polyprenyl pyrophosphate-glucose. The glucolipid is labile to mild acid hydrolysis; when chromatographed in alkaline solvents, glucose-1,2-cyclic phosphate is obtained. The pattern elution of a DEAE-cellulose column fits in well with a pyrophosphate derivative. Exogenous polyprenyl phosphate stimulated the incorporation of glucose into lipids; when incubated with (.beta.-32P)-UDP-glucose, radioactivity is incorporated into lipids and the reaction is reversed by 5 mM UMP. It is suggested that the glucolipid is an intermediate in glycan biosynthesis.