Polyamines Stimulate Suppression of Amber Termination Codons in vitro by Normal tRNAs

Abstract
Polyamines, such as spermine and spermidine, are able to stimulate reading of amber termination codons on viral messenger RNAs in vitro. This phenomenon is not due to an overall increase of error frequency during translation, but to a specific effect on a normal tRNA that is present in various eukaryotic cell preparations. The enhancement of reading of termination codons by normal tRNAs should be of major importance for the expression of specific genes in eukaryotic cells.