ADrosophilagene structurally and functionally homologous to the mammalian 70-kDa S6 kinase gene
- 26 November 1996
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 93 (24) , 13694-13698
- https://doi.org/10.1073/pnas.93.24.13694
Abstract
CDNAs encoding theDrosophila70-kDa S6 kinase (S6K) were isolated by low-stringency hybridization with mammalian p70S6kprobes. Conceptual translation ofS6kcDNA sequences yields a product containing all of the canonical features typical of serine/threonine kinases and has 78% amino acid identity in the catalytic domain with the human p70S6khomologue. TheS6kgene, located at polytene chromosome site 65D, gives rise to two predominant transcripts of 3.0 and 5.0 kb and at least two smaller transcripts (RpS6air8) mutant animals express higher levels of the smallerS6ktranscripts, suggesting tissue- or genotype-specific differences in the regulation of theS6kgene.DrosophilaS6K expressed in COS or NIH 3T3 cells phosphorylates mammalian RPS6 in a mitogen-dependent wortmannin- and rapamycin-sensitive manner, suggesting that its regulation is similiar to mammalian p70S6k.Keywords
This publication has 36 references indexed in Scilit:
- The 70 kDa S6 Kinase Complexes with and Is Activated by the Rho Family G Proteins Cdc42 and Rac1Cell, 1996
- Cell Cycle Regulation of p70 S6 Kinase and p42/p44 Mitogen-activated Protein Kinases in Swiss Mouse 3T3 FibroblastsPublished by Elsevier ,1996
- The 70 kDa S6 kinase: regulation of a kinase with multiple roles in mitogenic signallingCurrent Opinion in Cell Biology, 1995
- Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase BNature, 1995
- The Phosphodiesterase Inhibitor SQ 20006 Selectively Blocks Mitogen Activation of p70S6k and Transition to S Phase of the Cell Division Cycle without Affecting the Steady State Phosphorylation of eIF-4EPublished by Elsevier ,1995
- Rapamycin, Wortmannin, and the Methylxanthine SQ20006 Inactivate p70s6k by Inducing Dephosphorylation of the Same Subset of SitesPublished by Elsevier ,1995
- Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transductionNature, 1995
- Differential Subcellular Localization of Two Isoforms of p70 S6 Protein KinaseBiochemical and Biophysical Research Communications, 1994
- p70s6k function is essential for G1 progressionNature, 1993
- The Protein Kinase Family: Conserved Features and Deduced Phylogeny of the Catalytic DomainsScience, 1988